CENTRE FOR ANIMAL HEALTH STUDIES

PHARMACOVIGILANCE LABORATORY FOR ANIMAL FEED AND FOOD SAFETY (PLAFFS)

VISION

To prepare and meet out WTO Challenges pertaining to xenobiotic residues (Veterinary drugs, Pesticides and Mycotoxins)
To establish poison control information service

OBJECTIVES

Short-Term Objectives

To have a constant pharmacovigilance on Veterinary drugs (Antibiotics, growth promoters, anthelmenitics) - Drugs which have been banned for use in food animals and Drugs (Antibiotics/Antimicrobials) with MRL

To analyze mycotoxins, pesticides, insecticides, herbicides, minerals, heavy metals in dry and wet samples.

To generate a database on the spectrum of veterinary drugs in feed and feedstuff in Tamil Nadu.

Long-Term Objectives

Traceability /sources of Point contamination
Postmarket surveillance of approved veterinary drugs in livestock products

Quality Policy

Pharmacovigilance Laboratory for Animal Feed and Food Safety (PLAFFS) functioning under the Directorate, Centre for Animal Health Studies (CAHS), Tamil Nadu Veterinary and Animal Sciences University (TANUVAS) is to offer the facility of testing and reporting the levels of Mycotoxins, Drug residues, Pesticides from various feeds, feed stuff and livestock farmers to prepare their products as per International Standards. The service of this laboratory is open to continuous improvement based on feedbacks and findings and through the application of the Quality Policy and Quality objectives.

Salient Findings

T2 toxicosis in ostriches - 2000
Organophosphorous toxicity – 2000
Nitrate/Nitrite toxicosis in equines-2001
Zinc phosphide toxicity in monkeys-2005
Hydrocyanic acid toxicity in cattle-2009
Nitrate/Nitrite toxicity (Weeds) in ponies and cattle-2009
Organocarbamate – Carbofuran toxicity in elephants-2009
Tetradeconic acid toxicity in heifers-2010
Dimethyl pentane in Tilopia fish - 2017
Malathion and Carbofuran toxicity in Broiler Feed - 2017
Glyphosate (Herbicide) toxicity in chicken - 2018
Acetamprid (Pesticide) toxicity in Fish - 2018
Ricinoleic acid and Zinc Phosphide toxicity in cattle feed and feed ingredients - 2018
Glyphosate (Herbicides) toxicity in pelican birds - 2023
Thiram/Thiuram disulfide (Fungicide) in maize - 2023
Metalochlor and Pretachlor (Weedicides) in feed ingredients like maize and soya - 2024

Test Conducted

Analysis of animal feed and food for the presence of the following is being carried out:

Mycotoxins – Aflatoxin, Ochratoxin, Citrinin, Penicillic acid, T2 Toxin, Zearalenone
Pesticides – Organochlorine, Organophosphorous Carbamate & Pyrethroids
Veterinary drugs - Tetracycline, Chloramphenicol, Nitrofurans and Enroflaxacin
Adulterants – Urea
Pharmacotoxicological diagnosis in disease outbreak (Nitrate/Nitrite, Zinc Phosphide, Urea, Hydrocyanic Acid, Gossypol, Ricinoleic acid)

Facilities Available/ Services Offered

Sl. No	Name of the Parameter	Detection method	Existing Rate (Rs.)			Proposed Rate (Rs.)
			Student	Tamil Nadu	Outside state	College Student						Tamil Nadu			Outside State
						TANUVAS			Other College			Tamil Nadu			Outside State
						Charges	18% GST	Total	Charges	18% GST	Total	Charges	18% GST	Total	Charges	18% GST	Total
1	AFLATOXINS (B1, B2, G1, G2, M1 and M2)	Thin Layer Chromatography (TLC)	250	300	350	350	No GST	350	450	81	531	600	108	708	700	126	826
1	AFLATOXINS (B1, B2, G1, G2, M1 and M2)	High Performance Thin Layer Chromatography (HPTLC)	800	1200	1300	900	No GST	900	1000	180	1180	1200	216	1416	1300	234	1534
2	MYCOTOXINS (Aflatoxins, Citrinin, Ochratoxin A, T2 Toxin, and Zearalenone)	Thin Layer Chromatography (TLC)	400	750	1000	600	No GST	600	700	126	826	900	162	1062	1000	180	1180
2		High Performance Thin Layer Chromatography (HPTLC)	800	1200	1300	1200	No GST	1200	1300	234	1534	1400	252	1652	1500	270	1770
3	PESTICIDES Organophosphates, Organocarbamates, and Pyrethroids	Thin Layer Chromatography (TLC)	300	400	500	1200	No GST	1200	1300	234	1534	1500	270	1770	1600	288	1888
	Organochlorines, Organophosphates, Organocarbamates, and Pyrethroids	Gas Chromatography Mass Spectrometry (GCMS) (Per compound)	750	1200	1300	1400	No GST	1400	1500	270	1770	1600	288	1888	1700	306	2006
		Gas Chromatography (GC) (Per compound)	800	1200	1300	1500	No GST	1500	1550	279	1829	1600	288	1888	1700	306	2006
4	VETERINARY DRUGS Chlortetracycline, Ciprofloxacin, Enrofloxacin, Salinomycin, Tiamulin, Tylosin, Furazolidone and metabolites	Thin Layer Chromatography (TLC)	300	400	500	700	No GST	700	800	144	944	900	162	1062	1000	180	1180
4		High Performance Liquid Chromatography (HPLC) (Per compound)	800	1200	1300	1200	No GST	1200	1300	234	1534	1400	252	1652	1500	270	1770
5	MINERALS (Calcium, Magnesium, Potassium, Sodium, Manganese, Zinc, Selenium, Phosphorus and Sulfur)	Inductively Coupled Plasma Mass Spectrometry (ICPMS)	1000	1500	2000	1500	No GST	1500	1600	288	1888	1800	324	2124	2100	378	2478
6	HEAVY METALS (Lead, Mercury, Cadmium, Arsenic, Chromium, Cobalt, Copper, Iron, Nickel, Boron, Aluminium, Silver, Bismuth, Barium, Lithium, Vanadium, Zirconium, Tin, Molybdenum, Antimony, Silicon and Gold)	Inductively Coupled Plasma Mass Spectrometry (ICPMS)	1000	1500	2000	1500	No GST	1500	1600	288	1888	2000	360	2360	2200	396	2596
7	Nitrate/Nitrite	Qualitative & Quantitative	300	600	1000	300	No GST	300	400	72	472	700	126	826	1000	180	1180
8	Urea	Qualitative & Quantitative	200	250	300	300	No GST	300	350	63	413	400	72	472	500	90	590
9	Zinc Phosphide	Qualitative	-	-	-	300	No GST	300	400	72	472	600	108	708	1000	180	1180
10	Ricinoleic acid	Gas Chromatography Mass Spectrometry (GCMS)	-	-	-	800	No GST	800	1000	180	1180	1200	216	1416	1300	234	1534
11	Hydrocyanic acid (HCN)	Qualitative	-	-	-	300	No GST	300	350	63	413	400	72	472	500	90	590
12	Gossypol	Qualitative	-	-	-	300	No GST	300	400	72	472	600	108	708	1000	180	1180
13	Water analysis	Quantitative	-	-	-	300	No GST	300	400	72	472	600	108	708	1000	180	1180

Instrument Charges

Sl. No.	Name of the Instrument	Charges (Rs.)	18% GST (Rs.)	Total (Rs.)
1	Thin Layer Chromatography (TLC)	500	90	590
2	UV Spectrophotometer	500	90	590
3	High Performance Thin Layer Chromatography (HPTLC)	800	144	944
4	High Performance Liquid Chromatography (HPLC)	800	144	944
5	Gas Chromatography (GC)	800	144	944
6	Gas Chromatography Mass Spectrometry (GCMS)	800	144	944
7	Inductively Coupled Plasma Mass Spectrometry (ICPMS)	1,800	360	2,160

Quantity of Sample required for Analysis

Sample	Quantity/ Numbers
Compounded feed / feed ingredients	250-500grams
Bio-specimens	Minimum 50grams in Saturated Saline
Milk	100ml
Meat	200-250grams
Egg	6-10 Numbers
Fodder	500grams

Faculty

Name and Designation	E-mail	Mobile#
Dr.S.Ezhil Valavan, Professor and Head	ezhilvalavan.s@tanuvas.ac.in	+91-9444852355
Dr.D.Raghu, Assistant Professor	raghu_horti@rediffmail.com	+91-9790498964

Professor and Head,
Pharmacovigilance Laboratory for Animal Feed and Food Safety,
Centre for Animal Health Studies, TANUVAS,
Madhavaram Milk Colony, Chennai – 600051.
Phone: +91-44-2555 0111
E-mail: plaffs@tanuvas.org.in