PHARMACOVIGILANCE LABORATORY FOR ANIMAL FEED AND FOOD SAFETY

(PLAFFS)

Vision

  • To prepare and meet out WTO Challenges pertaining to xenobiotic residues (Veterinary drugs, Pesticides and Mycotoxins).
  • Labeling of Livestock foods GMO/Non GMO
  • To establish poison control information service
  • To obtain NABL accreditation 17025

Objectives

Short Term Objectives

  • To have a constant pharmacovigilance on Veterinary drugs (Antibiotics, growth promoters, anthelmenitics)
    • Drugs which have been banned for use in food animals
    • Drugs (Antibiotics/Antimicrobials) with MRL
  • To generate a database on the spectrum of veterinary drugs in feed and feedstuff in Tamil Nadu.

Long Term Objectives

  • Traceability /sources of Point contamination.
  • Postmarket surveillance of approved veterinary drugs in livestock products.

Quality Policy

Pharmacovigilance Laboratory for Animal Feed and Food Safety (PLAFFS) functioning under the Directorate, Centre for Animal Health Studies (CAHS), Tamil Nadu Veterinary and Animal Sciences University (TANUVAS) is to offer the facility of testing and reporting the levels of Mycotoxins, Drug residues, Pesticides and GMO's from various feeds, feed stuff and livestock farmers to prepare their products as per International Standards.
The service of this laboratory is open to continuous improvement based on feedbacks and findings and through the application of the Quality Policy and Quality objectives.

Salient Findings

  • T2 toxicosis in ostriches - 2000
  • Organophosphorous toxicity – 2001
  • Nitrate/Nitrite toxicosis in equines-2001
  • Urea toxicity in heifers-2002
  • Nitrate/Nitrite toxicosis in cattle-2004
  • Zinc phosphide toxicity in monkeys-2005
  • Hydrocyanic acid toxicity in cattle-2005
  • Nitrate/Nitrite toxicity (Weeds) in ponies and cattle-2008
  • Hydrocyanic acid toxicity (Tapioca) in cattle-2009
  • Organocarbamate – Carbofuran toxicity in elephants-2009
  • Tetradeconic acid toxicity in heifers-2010

Facilities Available at PLAFFS as per European Union / FDA Norms

High Performance Thin Layer chromatography (HPTLC) for Screening of Mycotoxins, Pesticides and Veterinary Drugs.
High performance Liquid Chromatography (HPLC) for identifying Mycotoxins
Gas Chromatography (GC) for identifying Pesticides
Gas Chromatography Mass Spectrometer (GC MS) for molecular fragmentation and confirmation of Melamine and Pesticides.

Facilities Available at PLAFFS as per European Union / FDA Norms

Liquid Chromatography Mass Spectrometer (LC MS MS) for molecular fragmentation and confirmation of Veterinary drugs.
Automated Gel Permeation Chromatography (GPC) for cleanup of samples prior to LC MS MS/GC MS identification.
PCR Based Microarray for Detection of GMO'S first of its kind in the country for screening livestock products.

Test Conducted

  • Analysis of animal feed and food for the presence of the following is being carried out
    • Mycotoxin – Aflatoxin, Ochratoxin, Citrinin, Penicillic acid, T2 Toxin, Zearalenone
    • Pesticides – Organochlorine, Organophosphorous & Pyrethroids
    • Veterinary drugs
      • Banned Veterinary drugs – Chloramphenicol, Nitrofurans, Sulphanamides & Enroflaxacin
      • Drugs with permissible level – Tetracycline, Macrolide & Betalactam
    • Adulterants – Melamine/Urea
  • Screening for GMO in Feed/Livestock Products
  • Pharmacotoxicological diagnosis in disease outbreak (Nitrate/Nitrite, Zinc Phosphide, Urea toxicity, Hydrocyanic Acid toxicity)

Present Analytical Tariff

Name of the Parameter Detection method Students Charges/ Test (Rs.) Tamil Nadu Charges/ Test (Rs.) Outside state Charges/ Test (Rs.)
PESTICIDES
Organocarbamates
Organochlorine
Organophosphates,
Pyrethroids--
Thin Layer Chromatography (TLC) 300 400 500
Gas Chromatography (GC) per Compound 800 1200 1300
Gas Chromatography Mass Spectrometer (GCMS) per Compound 750 1200 1300
VETERINARY DRUGS
Chlortetracycline Ciprofloxacin Enrofloxacin Salinomycin, Tiamulin, Tylosin Thin Layer Chromatography (TLC) 300 400 500
High Performance Liquid Chromatography (HPLC) per Compound 800 1200 1300
Chloramphenicol , Furazolidone and metabolites Liquid Chromatography Mass Spectrometer Mass Spectrometer (LCMS/MS) per Compound 1000 1500 2000
MYCOTOXINS
Aflatoxins Multimycotoxins Thin Layer Chromatography (TLC) 250 300 350
(Ochratoxin A, Citrinin, T2 toxin, Penicillic acid, Zearalenone, Patulin and Rubratoxin)- High Performance Thin Layer Chromatography (HPTLC) per Compound 800 1200 1300
High Performance Liquid Chromatography (HPLC) per Compound 800 1200 1300
Genetically Modified Foods Detection (GMO) PCR Based Micro Array 5000 per sample 8000 per sample 10000 per sample
Nitrate/ Nitrite - 300 600 1000
Urea - 200 250 300
PESTICIDES
Organocarbamates
Organochlorine
Organophosphates,
Pyrethroids
Thin Layer Chromatography (TLC) 300 400 500
GC Gas Chromatography (GC) per Compound 800 1200 1300
GCMS Gas Chromatography Mass Spectrometer (GCMS) per Compound 750 1200 1300
VETERINARY DRUGS
Chlortetracycline
Ciprofloxacin
Enrofloxacin ,
Salinomycin,
Tiamulin ,
Tylosin
Thin Layer Chromatography (TLC) 300 400 500
High Performance Liquid Chromatography (HPLC) per Compound 800 1200 1300
Chloramphenicol , Furazolidone and metabolites Liquid Chromatography Mass Spectrometer Mass Spectrometer (LCMS/MS) per Compound 1000 1500 2000
MYCOTOXINS
Aflatoxins (B1, B2, G1, G2, M1 and M2) Thin Layer Chromatography (TLC) 250 300 350
Multi mycotoxins Thin Layer Chromatography (TLC) 400 750 1000
(Ochratoxin A, Citrinin, T2 toxin, Penicillic acid, Zearalenone, Patulin and Rubratoxin) Thin Layer Chromatography (TLC) 200 250 300
HPTLC High Performance Thin Layer Chromatography (HPTLC) per Compound 800 1200 1300
HPLC High Performance Liquid Chromatography (HPLC) per Compound 800 1200 1300
Genetically Modified Foods Detection (GMO) PCR Based Micro Array 5000 per sample 8000 per sample 10000 per sample
Nitrate/ Nitrite - 300 600 1000
Urea - 200 250 300
FTIR Fourier transform infrared spectroscopy per compound 150 200 300
ICPMS Inductively coupled plasma mass spectrometry per compound 1000 1500 2000
LCMS - Time of Flight Liquid chromatography mass spectrometer per compound 1000 1500 2000

 

Quantity of Sample to be Sent for Analysis

  • Compounded feed / feed ingredients : 250-500g
  • Bio-specimens: Minimum 50g in saturated saline
  • Milk : 100mL
  • Meat : 200-250g
  • Egg : 6-10 nos
  • Fodder : 500g

Trainings Offered

  • Inplant training on "Chromatographic Techniques & Mycotoxins – Purified by Chromatographic Techniques" from 14.05.2010-14.06.2010.
  • A one day awareness programme on "Residue and Quality Assurance of Feed and Livestock Products" as a part of World Veterinary Year-2011 celebrations was organized by Pharmacovigilance Laboratory for Animal Feed and Food Safety (PLAFFS), Directorate of Centre for Animal Health Studies, TANUVAS on 22.12.2011 at PLAFFS, Madhavaram Milk Colony, Chennai-51. Fourteen participants representing various districts of Tamil Nadu including 8 Assistant Directors from Animal Disease Investigation Unit, 1 Research Associate from Central Referral Laboratory, Department of Animal Husbandry and 5 Assistant Research Officers from Institute of Veterinary Preventive Medicine, Ranipet attended the programme.
  • B.Tech-inplant- Final year B.Tech (FPT) students, College of Food and Dairy Technology, Koduvalli from 13.03.2012 to 25.06.2012.
  • Project work entitled
    • "Incidence of pesticide residues and assessment of physico-chemical parameters in grape and its products"
    • "Incidence of aflatoxin contamination and assessment of physico-chemical parameters in breakfast cereals".
  • National workshop
    • A National Workshop on "Perspective in Veterinary Pharmacovigilance, Feed and Food Safety" was organised under the support of Drugs and Pharmaceutical Research Programme, Department of Science and Technology, Government of India, New Delhi from 23rd to 25th September 2010 at Pharmacovigilance Laboratory for Animal Feed and Food Safety, Directorate of Centre for Animal Health Studies, Madhavaram Milk Colony, Chennai-51. Eighteen delegates from various institutes, industries and entrepreneurs representing Andhra Pradesh, Gujarat, Haryana, Kerala, Karnataka, Madhyapradesh, Maharashtra and Tamil Nadu states registered for the Workshop.

Collaborative Ongooing Research

  • APEDA - Rs. 5.555 crores has been sanctioned by Agricultural and Processed Food Products Export Development Authority (APEDA), Government of India, New Delhi, for the project entitled "Strengthening of Pharmacovigilance Laboratory for Animal Feed and Food Safety under WTO norms for the export of Livestock Products"
  • TNAU-"Development of immunological and molecular tools for diagnosis and management of Aspergillus flavus infection and aflatoxin contamination in foods and feeds" budget - 24,60,000, funding agency - Government of India, Department of Science and Technology, Technology Bhawan, New Delhi -110016
  • NEOSPARK- "Development of novel mycotoxin binders for the management for mycotoxicosis in animal and human" DST Share – (70%)=Rs.12,60,000/- Neospark – (30%) =Rs. 5,40,000/- (as cash and kind) funding agency - GOI, DST, New Delhi.

Faculty

Dr. Ghadevaru Sarathchandra, Ph.D., Professor and Head | sarathchandra.g@tanuvas.ac.in
Dr. P. Pothiappan, Assistant Professor | pothiappan.p@tanuvas.ac.in
Dr. K. Vijayakaran, Assistant Professor | vijaykaran.k@tanuvas.ac.in
Dr. G. R. Athira, Graduate Assistant | athira.g.r@tanuvas.ac.in

For further details, contact:

The Professor and Head,
Pharmacovigilance Laboratory for Animal Feed and Food Safety,
Centre for Animal Health Studies,
Madhavaram Milk Colony, Chennai - 600 051.
Tele Fax: 044 - 2555 0111
e-mail: plaffs@tanuvas.org.in